CHARACTERISTICS OF THE MEASUREMENT OF DNA ADDUCTS BY THE ³²P POSTLABELING METHOD:

ADN’TOX allows access to its technological platform for DNA adduct measurement by 32P-postlabeling and its expertise in genotoxicology


CHARACTERISTICS OF THE 32P POSTLABELING METHOD

  • - Dosimetry of DNA impacts

  • - High sensibility of the measurement
    (limit of detection around 1 adduct in 10 exp 10 normal nucleotides)

  • Limited needs in biological material
    (around 10 µg DNA)

  • Detection of DNA adducts from several identified or unidentified chemicals

  • Possibility to use the method in a broad range of study methodologies (in vitro, in vivo, human)


ADN’TOX’s KNOW-HOW & EXPERIENCE

  • Studies in human field:
    Occupational exposures, biological monitoring, exposure-effect relationship, risk groups…

  • - Studies in Environment:
    Monitoring of environmental contaminations, biomarker development, genotoxic impact…

  • Mechanistic approaches
    Measurement of adducts in target and surrogate tissues, dose-effect relationship, risk assessment, complement in the usual battery of assays.